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KMID : 0545120090190010099
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 1 p.99 ~ p.107
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Aeration Control of Thermophilic Aerobic Digestion Using Fluorescence Monitoring
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Pragash Gandhi
Narayanan Badri
Naik Ravindra
SAKTHIVEL
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Abstract
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Strain PUPC1 produces an antifungal protease as well as plant growth promoting enzymes such as 1- aminocyclopropane-1-carboxylate (ACC) deaminase and phosphatase. Morphological, cultural, and physiological characteristics as well as 16S rRNA gene-sequence-based phylogenetic analysis confirmed the taxonomic affiliation of PUPC1 as Chryseobacterium aquaticum. The optimum growth of PUPC1 was observed at pH 6.0 and 30oC, and maximum protease production was observed in medium B amended with 1% tryptone, 0.5% sucrose, and 0.005% MnCl2. The protease was purified by ammonium sulfate precipitation, Sephadex G-75 gel filtration chromatography, and electroelution from preparative SDS-PAGE. The protease had a molecular mass of 18.5 kDa. The optimum pH and temperature stability of the protease were pH 5.0- 10.0 and temperature 40-70oC. Chryseobacterium aquaticum PUPC1 and its protease showed a broad-spectrum antifungal activity against phytopathogenic fungi. Strain PUPC1 also exhibited plant growth promoting traits. The objective of the present investigation was to isolate a strain for agricultural application for plant growth promotion and biocontrol of fungal diseases.
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KEYWORD
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Chryseobacterium aquaticum, protease, gel filtration chromatography, antifungal activity, phylogenetic tree
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